The DOI matches its Crossref record, with a small difference in title or year; 27 of the 27 references it cites were found in a registry or an index.
Identification, Sequence Analysis, and Comparative Study on GSTe2 Insecticide Resistance Gene in Three Main World Malaria Vectors: Anopheles stephensi, Anopheles culicifacies, and Anopheles fluviatilis
Abstract
Glutathione S-transferases (GSTs) are soluble dimeric proteins that are involved in the metabolism, detoxification, and excretion of a large number of endogenous and exogenous compounds such as insecticides from the cell. In the current study, field specimens of Anopheles stephensi Liston, Anopheles fluviatilis James, and Anopheles culicifacies Giles collected from Sistan and Baluchistan province in Iran and subjected to World Health Organization susceptibility test. Only An. stephensi was resistant to 4% DDT. DNA extraction and rDNA-ITS2-polymerase chain reaction (PCR) for correct species identification, followed by amplification of GSTe2 gene, including exon I and II and full sequence of intron I, identified a 500-bp fragment in these three species. These fragments were purified and sequenced from both ends. The comparison of coding sequence of GSTe2 gene between these species and with Anopheles gambiae Giles showed 82 to 86% similarity at nucleic acid levels and identified nucleotide polymorphisms within An. culicifacies and An. stephensi populations. Species-specific differences have been detected in intron I of GSTe2 gene. This is in concordance with the previous studies and confirmed the conserved nature of intron sequence in GSTe2 gene of each species, probably useful as a molecular marker for species-specific identification. Phylogenetic analysis based on rDNA-ITS2, and coding (exon I and II) and noncoding sequences of GSTe2, showed the systematic relatedness between…
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Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“DDT resistance in Iran and many other countries is primarily GST-related [43, 62, 84–86, 89].”
From Evolution of insecticide resistance and its mechanisms in Anopheles stephensi in the WHO Eastern Mediterranean Region (2020) · cites this paper as [62]
In the citing paper
“A genomic study on DDT-resistant strain of An. stephensi showed that GSTe2 may be an underlying resistance mechanism in DDT resistance in Iranian An. stephensi [62].”
From Evolution of insecticide resistance and its mechanisms in Anopheles stephensi in the WHO Eastern Mediterranean Region (2020) · cites this paper as [62]
In the citing paper
“Over the years, DDT resistance persisted but that of dieldrin decreased more rapidly changing its susceptibility status to tolerance [61], and later to complete susceptibility [62–64].”
From Evolution of insecticide resistance and its mechanisms in Anopheles stephensi in the WHO Eastern Mediterranean Region (2020) · cites this paper as [62]
In the citing paper
“Regardless of a couple of instances of mortality of less than 95% in 2011 [64] and 90% in 2015 [72], the species proved to be susceptible to malathion-discriminating concentration since 1982 in its entire range in southern Iran [19, 59–62, 65, 66, 68, 69, 71].”
From Evolution of insecticide resistance and its mechanisms in Anopheles stephensi in the WHO Eastern Mediterranean Region (2020) · cites this paper as [62]
In the citing paper
“Follow-up bioassays from 2004 to 2010 revealed complete susceptibility to these insecticides in An. stephensi from its entire range in southern areas of Iran [59–64, 66].”
From Evolution of insecticide resistance and its mechanisms in Anopheles stephensi in the WHO Eastern Mediterranean Region (2020) · cites this paper as [62]
Citations
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Abbasi, E., Vahedi, M., Bagheri, M., Gholizadeh, S., Alipour, H., & Moemenbellah‐Fard, M. D. (2022) · Heliyon · doi:10.1016/j.heliyon.2022.e08830 · Cited by 68
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Self-citationSeasonal Abundance and Host-Feeding Patterns of Anopheline Vectors in Malaria Endemic Area of Iran
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Self-citation
Metadata
- Authors
- Djadid, N. D., Barjesteh, H., Raeisi, A., Hassanzahi, A., & Zakeri, S.
- Affiliations
- Pasteur Institute of Iran (IR)
- Zahedan University of Medical Sciences (IR)
- Venue
- Journal of Medical Entomology
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-11-01
- Volume
- 43
- Issue
- 6
- Pages
- 1171-1177
- ISSN
- 0022-2585 (ISSN-L)
- OpenAlex
- W2180259388
- Cited by
- 17
- References
- 26
- Citations by year
- 2022: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Entomology (publishedVersion)
- PubMed (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glutathione Transferases and Polymorphisms
- Keywords
- GSTe2, glutathione S-transferase, insecticide resistance, DDT resistance, Anopheles stephensi, Anopheles culicifacies, Anopheles fluviatilis, malaria vectors, rDNA ITS2, species-specific identification, phylogenetic analysis, Anopheles gambiae, Sistan and Baluchestan
- MeSH terms
- Animals, Anopheles, Base Sequence, Glutathione Transferase, Insect Vectors, Insecticide Resistance, Iran, Molecular Sequence Data, Phylogeny, Plasmodium, Polymorphism, Genetic, Cluster Analysis, Sequence Alignment, Sequence Homology, Sequence Analysis, DNA, Toxicity Tests
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- CoChat. (2026). Citation check: Identification, Sequence Analysis, and Comparative Study on GSTe2 Insecticide Resistance Gene in Three Main World Malaria Vectors: Anopheles stephensi, Anopheles culicifacies, and Anopheles fluviatilis. https://app.cochat.ai/papers/10.1603/0022-2585(2006)43%5B1171:isaacs%5D2.0.co;2
- The paper
- Djadid, N. D., Barjesteh, H., Raeisi, A., Hassanzahi, A., & Zakeri, S. (2006). Identification, Sequence Analysis, and Comparative Study on GSTe2 Insecticide Resistance Gene in Three Main World Malaria Vectors: Anopheles stephensi, Anopheles culicifacies, and Anopheles fluviatilis. Journal of Medical Entomology. https://doi.org/10.1603/0022-2585(2006)43%5B1171%3Aisaacs%5D2.0.co%3B2
